Anti-Green Fluorescent Protein (GFP) Antibody GFP-1020 from Aves Labs, Inc.

Product Specs
ItemAnti-Green Fluorescent Protein (GFP) Antibody
CompanyAves Labs, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberGFP-1020
Antibody TypePrimary Antibody
ClonalityPolyclonal
FormatMixture of IgY fraction and affinity-purified antibodies, Liquid
HostChicken
Quantity400 µL
Molecular Weight27 kDa
TargetGreen Fluorescent Protein (GFP)
IsotypeIgY
Conjugate/TagUnconjugated
Brand/TrademarkAves Labs
ImmunogenRecombinant GFP expressed in Escherichia coli
ValidationAntibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY
Regulatory StatusThese antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans

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Aves Labs, Inc.

P.O. Box 1560

Davis, CA 95617-1560

United States

Phone: (800) 824-8540

Email: orders@aveslabs.com

Company Profile

Website: https://www.aveslabs.com/

(1) BMP7 expression in mammalian cortical radial glial cells increases the length of the neurogenic periodProtein & cellJanuary 3, 2024Li Z, Liu G, Yang L, Sun M, Zhang Z, Xu Z, Gao Y, Jiang X, Su Z, Li X, et al.11419-1-AP), rabbit anti-ID1 (1:2,000, Biocheck Inc, BCH-1/37-2), and chicken anti-GFP (1:3,000, Aves labs, GFP-1020). Mouse Bmp7 mRNA in situ hybridization Mouse Bmp7 mRNA in situ RNA hybridization experiment was performed

(2) Epilepsy-linked kinase CDKL5 phosphorylates voltage-gated calcium channel Cav2.3, altering inactivation kinetics and neuronal excitabilityNat CommunDecember 11, 2023Marisol Sampedro-Castañeda, Lucas L. Baltussen, André T. Lopes, Yichen Qiu, Liina Sirvio, Simeon R. Mihaylov, Suzanne Claxton, Jill C. Richardson, Gabriele Lignani, Sila K. UltanirAMR-006), anti GFP sdAb FluoTag-X4 (NanoTag Biochecnologies N0304or chicken anti GFP (1:1000, Aves GFP-1020). Secondary antibody incubation was for 1 h at RT. These were as follow: donkey anti rat Cy3 (Jackson

(3) CXCL12 targets the primary cilium cAMP/cGMP ratio to regulate cell polarity during migrationNat CommunDecember 4, 2023Melody Atkins, Maud Wurmser, Michèle Darmon, Fiona Roche, Xavier Nicol, Christine Métinantibody incubation. The following primary antibodies were used: chicken anti GFP (1/500, Aves Lab GFP-1020), rat anti RFP (1/1000, ChromoTek, clone 5F8), rabbit anti Arl13b (1/500, Proteintech 17711-1-AP), mouse

(4) PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1 ProteostasisBiomoleculesDecember 4, 2023Jason A. Kirkbride, Garbo Young Nilsson, Jee In Kim, Kosuke Takeya, Yoshinori Tanaka, Hiroshi Tokumitsu, Futoshi Suizu, Masumi EtoDanvers, MA, USA), anti-B-Raf (1:250, Merck #14-217, Darmstadt, Germany), anti-GFP (1:5000, Aves Lab #GFP1020, Davis, CA, USA), anti-HA (1:10,000, clone 12CA5), anti-CDK4 (1:2000, MyBioSource #MBS127817, San Diego,

(5) Multiple N-linked glycosylation sites critically modulate the synaptic abundance of neuroligin isoformsJournal of Biological ChemistryDecember 1, 2023Orion Benner, Thomas P. Cast, Laurie S. Minamide, Zephyr Lenninger, James R. Bamburg, Soham Chanda(1:1000 dilution; catalog no.: h3663; Sigma–Aldrich), or chicken anti-GFP (1:500 dilution; catalog no.: GFP-1020; Aves Labs), or mouse anti-GAPDH (1:50,000 dilution; catalog no.: 60004-1-Ig; Proteintech). The blots

(6) The subset of peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involvedPLoS OneDecember 1, 2023Tamara Somborac, Güleycan Lutfullahoglu Bal, Kaneez Fatima, Helena Vihinen, Anja Paatero, Eija Jokitalo, Ville O. Paavilainen, Svetlana Konovalovaimmunofluorescence analysis: rabbit anti-ACBD5, 1:300 (Atlas Antibodies, HPA012145), chicken anti-GFP, 1:10000 (Aves, GFP-1020), rabbit anti-catalase, 1:400 (CST, 12980S), rabbit anti-calnexin, 1:1000 (Abcam, 22595), rabbit anti-ATPIF1,

(7) Fibroblast growth factor 13-mediated regulation of medium spiny neuron excitability and cocaine self-administrationFrontiers in NeuroscienceNovember 30, 2023Nolan M. Dvorak, Jessica Di Re, Tileena E. S. Vasquez, Mate Marosi, Poonam Shah, Yorkiris M. Mármol Contreras, Matteo Bernabucci, Aditya K. Singh, Jariatu Stallone, Thomas A. Green, et al.solution +0.5% Triton for 1 h. Slices were stained with anti-GFP (Aves Lab, Davis, CA, United States GFP-1020) at 1:750. An isotype specific Alexa Fluor antibody was used the next day (anti-Chicken IgY 488, Invitrogen

(8) Pain and itch coding mechanisms of polymodal sensory neuronsCell ReportsNovember 28, 2023Changxiong Guo, Haowu Jiang, Cheng-Chiu Huang, Fengxian Li, William Olson, Weishan Yang, Michael Fleming, Guang Yu, George Hoekel, Wenqin Luo, et al.following antibodies and dyes were used: rabbit anti-GFP (Life Tech, A11122), chicken anti-GFP (Aves, GFP-1020), Alexa Fluor 488 conjugated IB4 (LifeTech, I21411), guinea pig anti-Vglut2 (EMD, AB2251), guinea pig

(9) Activation of KrasG12D in Subset of Alveolar Type II Cells Enhances Cellular Plasticity in Lung AdenocarcinomaCancer Research CommunicationsNovember 24, 2023Priyanka Chaudhary, Xia Xu, Guangfang Wang, Jacob P. Hoj, Rishi R. Rampersad, Marie-Liesse Asselin-Labat, Stephanie Ting, William Kim, Pablo Tamayo, Ann Marie Pendergast, et al.Millipore, #AB3786), rat anti-Rage (1:200, R&D Systems, #MAB1179), chicken anti-GFP (1:400, Aves Labs, #GFP1020), rabbit anti-Krt5 (1:500, Covance), rabbit anti-CC10 (1:10,000, Barry Stripp), rabbit anti-Sox2 (1:250,

(10) Synaptic mechanisms for associative learning in the cerebellar nucleiNature CommunicationsNovember 20, 2023Robin Broersen, Catarina Albergaria, Daniela Carulli, Megan R. Carey, Cathrin B. Canto, Chris I. De Zeeuwanti-GFP, which also detects YFP (1:1000, Chemicon, # AB3080), chicken anti-GFP (1:1000, Aves labs, GFP-1020), mouse anti-calbindin (1:1500, Swant, # 300), guinea-pig anti-VGLUT1 (1:500, Synaptic Systems, # 135304),

(11) Brn3b regulates the formation of fear-related midbrain circuits and defensive responses to visual threatPLOS BiologyNovember 20, 2023Hyoseo Lee, Hannah Weinberg-Wolf, Hae-Lim Lee, Tracy Lee, Joseph Conte, Carlos Godoy-Parejo, Jonathan B. Demb, Andrii Rudenko, In-Jung Kimwere: rabbit anti-GFP (1:1,000, Millipore, AB3080P), chicken anti-GFP (1:1,000, Aves Laboratories, GFP-1020), rabbit anti-cleaved caspase-3 (1:1,000, Cell Signaling Technology, 9661), rabbit anti-calbindin D-28K

(12) Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiencyCell Reports MethodsNovember 20, 2023Lei Jin, Heather A. Sullivan, Mulangma Zhu, Nicholas E. Lea, Thomas K. Lavin, Xin Fu, Makoto Matsuyama, YuanYuan Hou, Guoping Feng, Ian R. Wickershamthree days postinfection, using a 1:1000 dilution of chicken anti-GFP polyclonal antibody (Aves Labs, GFP-1020) and a 1:500 dilution of Alexa Fluor 594-conjugated donkey anti-chicken secondary antibody (Jackson Immuno

(13) Nucleus accumbens dopamine release reflects Bayesian inference during instrumental learningbioRxivNovember 17, 2023Albert J. Qü, Lung-Hao Tai, Christopher D. Hall, Emilie M. Tu, Maria K. Eckstein, Karyna Mishchanchuk, Wan Chen Lin, Juliana B. Chase, Andrew F. MacAskill, Anne G.E. Collins, et al.Immunohistochemistry was performed to amplify the GFP signal (1:1000 chicken anti-GFP, Aves Labs, Inc.; GFP-1020 followed by 1:1000 goat anti-chicken AlexaFluor 488, Invitrogen by Thermo Fisher Scientific; A11039).

(14) An adaptive stress response that confers cellular resilience to decreased ubiquitinationNature CommunicationsNovember 14, 2023Liam C. Hunt, Vishwajeeth Pagala, Anna Stephan, Boer Xie, Kiran Kodali, Kanisha Kavdia, Yong-Dong Wang, Abbas Shirinifard, Michelle Curley, Flavia A. Graca, et al.horse serum. The cells were then incubated overnight with primary antibodies (1:200) against GFP (Aves #GFP-1020) and PMP70 (abcam #ab3421) at 4 °C. Anti-FLAG antibodies (Cell Signaling #14793) were used in immunostaining

(15) Context-dependent regulation of Notch signaling in glial development and tumorigenesisScience AdvancesNovember 10, 2023Rongliang Guo, Danyu Han, Xingrui Song, Yanjing Gao, Zhenmeiyu Li, Xiaosu Li, Zhengang Yang, Zhejun Xugoat anti-EGFR (R&D Systems, AB-355937), rabbit anti-GFAP (Dako, Z0334), rabbit anti-GFP (Aves Labs, GFP-1020), rabbit anti-Glutamine synthetase (Oasis Biofarm, N2110151), rabbit anti-KI67 (Abcam, ab15580), rabbit

(16) Aryl hydrocarbon receptor restricts axon regeneration of DRG neurons in response to injurybioRxivNovember 10, 2023Yiqun Wang, Dalia Halawani, Molly Estill, Aarthi Ramakrishnan, Li Shen, Roland H. Friedel, Hongyan Zou5568S), anti-SCG10/STMN2 (rabbit, 1:1,000, Novus NBP1–49461), anti-GFP (chicken, 1:1,000, Aves Lab GFP-1020), anti-IBA1 (rabbit, 1:1,000, Wako 019–19741), anti-pSMAD1/5/8(rabbit, 1:1,000, Cell Signaling 9511), anti-GAP43(rabbit,

(17) A Massively Parallel Screen of 5′UTR Mutations Identifies Variants Impacting Translation and Protein Production in Neurodevelopmental Disorder GenesmedRxivNovember 9, 2023Stephen P. Plassmeyer, Colin P. Florian, Michael J. Kasper, Rebecca Chase, Shayna Mueller, Yating Liu, Kelli McFarland White, Courtney F. Jungers, Slavica Pavlovic Djuranovic, Sergej Djuranovic, et al.Rockland catalog no. 600–401-379) and chicken anti-eGFP primary antibody (1:1000, Aves Labs catalog no. GFP-1020). The sections were washed three times for five minutes before staining with donkey anti-rabbit Alexa

(18) High temporal frequency light response in mouse retina is mediated by ON and OFF bipolar cells and requires FAT3 signalingbioRxivNovember 9, 2023Evelyn C. Avilés, Sean K. Wang, Sarina Patel, Shuxiang Shi, Lucas Lin, Vladimir J. Kefalov, Lisa V. Goodrich, Constance L. Cepko, Yunlu XueTdTomato, 1:1,000, Clontech, Cat#632496), mouse anti-FAT36,7 (1:200), chicken anti-GFP (1:500; Aves, Cat#GFP-1020), mouse anti-GRIK1 (GluR5, 1:200, Santa Cruz Biotechnology, Cat#sc-393420)25, sheep anti-GRM6 (1:2,000,

(19) A neural mechanism for learning from delayed postingestive feedbackbioRxivNovember 9, 2023Christopher A. Zimmerman, Alejandro Pan-Vazquez, Bichan Wu, Emma F. Keppler, Eartha Mae Guthman, Robert N. Fetcho, Scott S. Bolkan, Brenna McMannon, Junuk Lee, Austin T. Hoag, et al.h, 2 h, overnight). CGRP stim timepoint samples also received primary (chicken anti-GFP, 1:500; Aves GFP-1020) and secondary (Alexa Fluor 594 donkey anti-chicken, 1:500; Jackson Immuno 703–585-155) antibodies for

(20) Generation of Human Blood Vessel and Vascularized Cerebral OrganoidsBIO-PROTOCOLNovember 5, 2023Xin-Yao Sun, Xiang-Chun Ju, Hong-Fang Zhao, Zhi-Wen You, Run-Run Han, Zhen-Ge Luo1:500)Goat anti-DCX (Santa Cruz, catalog number: sc-8066, 1:200)Chicken anti-GFP (Aves Lab, SKU number: GFP-1020, 1:1,000)Rabbit anti-IBA1 (Wako, catalog number: 019-19741, 1:500)DAPI fluorochrome (Beyotime, catalog

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Anti-Green Fluorescent Protein (GFP) Antibody from Aves Labs, Inc.

Aves Labs, Inc.

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Anti-Green Fluorescent Protein (GFP) Antibody from Aves Labs, Inc.

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